Problems of Forensic Sciences 2013 Vol. 96 (XCVI) 702-715
A STUDY OF SINGLE NUCLEOTIDE POLYMORPHISM IN THE MC1R GENE
Patrycja KULWIKOWSKA1, Krzysztof TALARCZYK2, Ryszard PAWŁOWSKI2
1Department of Obstetrics, Medical University of Gdańsk, Gdańsk, Poland
2Department of Forensic Medicine, Medical University of Gdańsk, Gdańsk, Poland
Streszczenie
Prediction of appearance based on genetic analysis is a very attractive prospect for forensic science. That is why trends towards researching those areas of DNA that are or may be highly associated with the phenotypic appearance of a person (pigmentation of the skin, hair and eye colour, height or body type) are more and more clearly visible in forensic genetics. It has been known for over 15 years that red hair colour is mainly related to variability in the gene of melanocortin receptor I. However, there have been no studies on the polymorphism of this gene in the northern Polish population. This paper presents a polymorphism study of the MC1R gene in a population of 100 persons of both sexes from this area. Hairs were analyzed by optical microscopy, assessing their morphological characteristics. DNA was isolated from mouth swabs and hair roots using the organic method. DNA was amplified using primers that are complementary to the genes of MC1R and amelogenin in multiplex PCR reactions. PCR products were minisequenced using appropriate primers. Eighteen people out of 95 had reddish hair colour. The relationship between SNP polymorphisms and the occurrence of reddish hair colour was examined.
Słowa kluczowe
MC1R; SNP polymorphism; RHC phenotype; Red hair colour.