Problems of Forensic Sciences 2013 Vol. 96 (XCVI) 740-751
PRELIMINARY DEVELOPMENT OF METHODOLOGY FOR THE ANALYSIS OF ORAL CAVITY FLUID FOR SELECTED PSYCHOTROPIC DRUGS
Agnieszka MOOS1, Renata WIETECHA-POSŁUSZNY1, Michał WOŹNIAKIEWICZ1, Viktoria BRODE1, 2, Paweł KOŚCIELNIAK1, 3
1Faculty of Chemistry, Jagiellonian University, Kraków, Poland
2Faculty of Chemistry and Mineralogy, University of Leipzig, Leipzig, Germany
3Institute of Forensic Science, Kraków, Poland
Streszczenie
The work is concerned with the development of methods for the analysis of alternative biological material – fluid from the oral cavity, for tricyclic psychotropic drugs (TCAs): imipramine, doxepin, nortriptyline, desipramine, nordoxepin and clomipramine. The oral cavity fluid samples were prepared for analysis using only the single step of centrifugation at a reduced temperature (5 min, 16,000 rpm, 4°C). Measurements were performed using a capillary electrophoresis system with spectrophotometric detection in the ultraviolet light range (UV-CE). The chemical and instrumental conditions of the method were optimized and its validation parameters were determined. It was shown that using the developed procedure it is possible to detect the studied drugs at concentrations of several tens of ng/ml, determine them with an average precision of about 10% (RSD) and with a satisfactory recovery level (except for clomipramine). The obtained results were compared with those obtained by liquid chromatography coupled with spectrophotometric detection (HPLC-UV), with the use of microextraction by packed sorbent (MEPS). To the authors’ best knowledge, this study is the first described example of an effective direct analysis of oral cavity fluid samples by CE without a prior stage of TCA drugs extraction from the biological matrix.
Słowa kluczowe
Fluid from oral cavity; TCAs; Capillary electrophoresis; HPLC-UV; Microextraction MEPS