Problems of Forensic Sciences 2009 Vol. 77 (LXXVII) 53-63
LC-MS AND LC-MS/MS DETERMINATION OF SIX ANTICOAGULANT RODENTICIDES IN BLOOD
Piotr ADAMOWICZ, Maria KAŁA
Institute of Forensic Research, Krakow, Poland
Streszczenie
Sensitive and reliable liquid chromatographic-mass spectrometric methods (LC-MS and LC-MS/MS) were developed for the determination of anticoagulants in whole blood. Six coumarin derivative anticoagulant rodenticides – bromadiolone, brodifacoum, difethialone, difenacoum, warfarin, and coumatetralyl – can be detected, identified and quantified simultaneously with this method. The method involves a weak acid (pH 5.5) liquid-liquid extraction with a mixture of chloroform-acetone. It allows separation of anticoagulants from each other and from major background compounds. Selected ion recording (SIR) and multiple-reaction monitoring (MRM) were used for the monitoring of negative ions for the detection of analysed compounds. Nimesulide was used as an internal standard (IS). The method is specific for analysis of post-mortem blood samples and blood samples taken from living persons suspected of having been poisoned by these compounds.
Słowa kluczowe
Anticoagulant rodenticides; Analysis; LC-MS.