Problems of Forensic Sciences 2006 Vol. 66 (LXVI) 165-171
DETERMINATION OF AMPHETAMINES IN BLOOD BY ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) IN THE PRACTICE OF THE INSTITUTE OF FORENSIC RESEARCH
Ewa JANOWSKA, Piotr ADAMOWICZ, Ewa CHUDZIKIEWICZ, Wojciech LECHOWICZ
Institute of Forensic Research, Krakow, Poland
Streszczenie
Methods based on immunochemical reactions are the most useful screening methods used in laboratories. The immunosorbent test kit ELISA (enzyme-linked immunosorbent assay) used for identification of compound groups in analysed materials with use of a drug-enzyme conjugate is classified into this group. The ELISA method was applied for routine analysis of blood and urine samples for the presence of drugs in the Institute of Forensic Research in 2006. Absorbance values less than or equal to absorbance of 50 ng/ml calibrator were taken as a positive result. All positive results were confirmed by the liquid chromatography-mass spectrometry (LC-MS) method. More than 11% of positive results were obtained by means of the ELISA method, in which the presence of amphetamine was confirmed by the LC-MS method in the concentration range from 57 to 583 ng/ml.
Słowa kluczowe
Blood; Amphetamine; ELISA; LC-MS.