Problems of Forensic Sciences 2005 Vol. 64 (LXIV) 373-381
SIMPLE HPLC METHOD FOR THE IDENTIFICATION OF THE MOST COMMONLY USED RODENTICIDES IN POLAND
Piotr ADAMOWICZ, Maria KAŁA
Institute of Forensic Research, Krakow
Streszczenie
A simple and reliable high-performance liquid chromatographic method with diode array detection (HPLC-DAD) was developed for the identification of anticoagulants in the most commonly used commercial rodenticide preparations in Poland. Six coumarin derivative anticoagulant rodenticides – bromadiolone, brodifacoum, difethialone, difenacoum, warfarin, and coumatetralyl – can be identified simultaneously with this method. The method involves weakly acid (pH 5.5) liquid-liquid extraction with a mixture of chloroform-acetone. This allows separation of anticoagulants from each other and from major background compounds. The procedure was applied to the identification of active anticoagulant components in rodenticide preparations and foodstuffs (soups, sausages and ground coffee) to which a rodenticide had been added for criminal purposes. Although the procedure was not developed for biological materials, it was also successfully utilised to analyse stomach content taken from animals (dogs, seals, and spoonbills) suspected of having been poisoned by these compounds.
Słowa kluczowe
Anticoagulant rodenticides; Analysis; HPLC-DAD.