Problems of Forensic Sciences 2004 Vol. 57 (LVII) 81-87
CHARACTERISATION OF NEW TETRANUCLEOTIDE POLYMORPHISM OF THE STR D5S1462 LOCUS
Dorota MONIES, Marzanna CIESIELKA, Roman MĄDRO
Chair and Department of Forensic Medicine, Medical Academy, Lublin
Streszczenie
The paper characterises a new tetranucleotide D5S1462 system (GDB: 08448). Genotyping of the system was performed in 102 unrelated individuals and in 50 trios (father – mother – child). Seven alleles were detected and sequenced using the automatic sequencer ALFexpress II. The sequence analysis revealed the presence of a complex repetitive sequence (ATCT)n (ATCC)n ATCT. The distribution of genotype frequencies was found to be consistent with the Hardy-Weinberg equilibrium. The calculated values of mean exclusion chance (MEC), power of discrimination (PD), polymorphism information content (PIC) and heterozygosity were 0.521, 0.895, 0.705 and 0.755, respectively.
Słowa kluczowe
Short tandem repeat; D5S1462; Allelic ladder; Sequencing; Population data.