Problems of Forensic Sciences 2004 Vol. 57 (LVII) 88-99
HVI MITOCHONDRIAL DNA DATA IN A POPULATION SAMPLE FROM SOUTH-EAST POLAND
Dorota MONIES, Marzanna CIESIELKA, Roman MĄDRO
Chair and Department of Forensic Medicine, Medical Academy, Lublin, Poland
Streszczenie
A population database was generated from 106 unrelated individuals living in South-East Poland. Mitochondrial DNA from hypervariable region I was determined by PCR amplification and direct sequencing. Sequence comparison led to the identification of 85 mitochondrial lineages, as defined by 67 variable positions. The most frequently occurring lineage comprised 11 individuals, whilst 57 types were observed in only one individual. A statistical analysis of the results for this population showed a genetic diversity of 0.94. The probability of two random individuals showing identical mtDNA haplotypes is 0.06. The average number of nucleotide differences between individuals is 3.74 bp.
Słowa kluczowe
Mitochondrial DNA; HVR-I; Polymerase chain reaction; Population study; Automatic sequencing.