Problems of Forensic Sciences 2001 Vol. 48 (XLVIII) 74-82
ALLELE FREQUENCIES OF HIGHLY POLYMORPHIC LOCUS D8S1132 IN A POPULATION SAMPLE FROM NORTH POLAND
Ryszard PAWŁOWSKI1,2, Marta ŻYWICKA3
1Chair and Department of Forensic Medicine, Medical Academy, Gdańsk
2Institute of Forensic Research, Cracow
3Intercollegiate Faculty of Biotechnology, Medical Academy, Gdańsk, and University of Gdańsk
Streszczenie
This paper presents the alleles frequency distribution for the D8S1132 locus in a population sample of 158 unrelated individuals of both sexes living in north Poland. PCR products were identified on a horizontal polyacrylamide gel stained with silver. The presence of the D8S1132*15 allele, which had not been observed till now, was ascertained using capillary electrophoresis on an ABI310 DNA sequencer. In the analysed population sample, the presence of 10 D8S1132 alleles was observed. Owing to the very high discrimination power (0.9622) of the analysed locus, it is highly useful for forensic identification purposes.
Słowa kluczowe
D8S1132 locus; STR; PCR; Population genetics; North Poland.