Problems of Forensic Sciences 2001 Vol. 47 (XLVII) 328-332
SUPERCRITICAL FLUID EXTRACTION OF TETRAHYDROCANNABINOL FROM MARIHUANA. DETERMINATION OF TETRAHYDROCANNABINOL AND TETRAHYDROCANNABINOL ACID
Lél EÖRY1, Vera SZALAY1, Tibor VERESS2
1Eötvös L. University, Department of Plant Anatomy, Budapest, Hungary
2Institute for Forensic Sciences, Budapest, Hungary
Streszczenie
A two-stage sample preparation procedure was elaborated for the analysis of THC (delta-9-tetrahydrocannabinol) and THC-acid in marihuana samples which includes in the first step the supercritical fluid extraction (SFE) of THC and in the second step the thermal decarboxylation1 of THC-acid to THC at conditions of static SFE and the subsequent extraction of THC formed. The THC and THC equivalent to the THC-acid content of the sample were determined by high performance liquid chromatographic (HPLC) analysis of extracts obtained in the first and second extraction step, respectively. The applicability of the proposed procedure is demonstrated by results obtained by analysis of different marihuana samples.
Słowa kluczowe
SFE; Marihuana; THC; THC-acid.