Problems of Forensic Sciences 2001 Vol. 45 (XLV) 66-80
GENETIC DATA ON NINE COMPLEX STR SYSTEMS IN THE POPULATION OF SOUTH-EASTERN POLAND AND THEIR USEFULNESS IN PATERNITY TESTING
Piotr KOZIOŁ, Marzanna CIESIELKA, Roman MĄDRO
Chair and Department of Forensic Medicine, Medical Academy, Lublin
Streszczenie
Population studies on 9 short tandem repeat (STR) systems (FGA, TPOX, D8S1179, VWA, Penta E, D18S51, D21S11, THO1, D3S1358) were conducted on a sample group of 317 unrelated residents of south-eastern Poland. Genotyping was based on a multiplex PCR reaction using the Promega GenePrint PowerPlex 2.1 system. Electrophoretic separation of the products was done in polyacrylamide denaturing gels, and an FMBIO II was used for detection of fluorochrome-marked fragments. The distribution of phenotypes and frequency of alleles are presented in the paper. These were compared to those of populations in other regions of Poland. A lack of significant deviations from the Hardy-Weinberg equilibrium (HWE) was observed, and all of the PowerPlex 2.1 markers proved highly informative, though they display largely varied polymorphism information content (PIC) values. Mean exclusion chance (MEC), mean paternity exclusion probability (MEP), and mean paternity index (MPI) values were considerable, and these studies can be expected to prove decisive in paternity cases. Results from the examination of 9 STR systems were compared with those obtained analysing 4 VNTR loci (D4S139, D5S110, D7S21, D12S11) in 63 paternity cases. Paternity was confirmed in 50 cases; a paternity index value (PI) of more than 10 000 was found in 37 of these, using the STR system, and in 42 cases using theVNTRsystem. In 48 cases, the combined PowerPlex 2.1 and 4 VNTR investigations provided a probability of paternity exceeding 99.99%, which is equivalent to certain paternity.
Słowa kluczowe
Nine STRs; Population Database; Linkage Equilibrium; Forensic genetics; Paternity testing.