Problems of Forensic Sciences 2000 Vol. 44 (XLIV) 24-42
ENANTIOSELECTIVE METHOD FOR DETERMINATION OF AMPHETAMINE AND ITS EIGHT ANALOGUES USING CHIRAL STATIONARY PHASE
Piotr ADAMOWICZ, Maria KAŁA
Institute of Forensic Research, Cracow
Streszczenie
Amphetamine is one of the most frequently abused drugs today. Like many other chiral compounds, amphetamine and its analogue enantiomers exhibit different activities, toxicities and behavioural effects, d-amphetamine being much more potent in its stimulating action than the l-form. There are medications on the market which contain only the l-enantiomer of methamphetamine (e.g. Vick Nasal Inhaler) or which metabolise to the l-enantiomers of methamphetamine or amphetamine (e.g. selegiline). To differentiate between the intake of these medicaments and abuse of amphetamine and methamphetamine, it is necessary to develop an enantioselective method of determination. The enantioseparation of amphetamine, methamphetamine and various other analogues of amphetamine by use of the chiral stationary phase, consisting of immobilised native β-cyclodextrin selectors, is presented. This method was elaborated for separation of specified compounds without any derivatisation. In this method 1-PEA and 2-PEA were used as internal standards for urine or non-biological materials analysis. Additionally, twelve different samples of amphetamine confiscated by police were analysed with respect to their enantiomeric composition.
Słowa kluczowe
Amphetamine; Amphetamine analogues; Enantiomers; HPLC; Chiral analysis.