Problems of Forensic Sciences 2000 Vol. 43 (XLIII) 275-282
DISTRIBUTION OF HYDROGEN SULPHIDE IN RATS' ORGANS AND ASSOCIATED HISTOLOGICAL CHANGES IN EXPERIMENTAL INTOXICATION
Roman WACHOWIAK1, Jarosław TOBOLSKI1, Bogdan MIŚKOWIAK2
1Department of Forensic Medicine, Medical Academy, Poznań, Poland
2Department of Histology and Embriology, Medical Academy, Poznań, Poland
Streszczenie
Chemistry-based diagnosis in cases of sudden death due to unnatural causes in cases of anoxemic deaths (e.g. in sewage collecting reservoirs) requires the possibility to estimate hydrogen sulphide levels in autopsy material. The usefulness of the analytical techniques spectrophotometric methods and gas chromatography "headspace" techniques with a thermal conductivity detector (TCD) for identification and determination of hydrogen sulphide in biological material were evaluated. Hydrogen sulphide determinations in biological samples taken from rats lethally intoxicated with gaseous hydrogen sulphide, permitted the selection of the most suitable autopsy material needed for routine toxicological analyses and its histological assessment.
Słowa kluczowe
Hydrogen sulphide; UV-VIS spectrometry; Gas chromatography.