Problems of Forensic Sciences 2000 Vol. 41 (XLI) 27-36
ISOCITRATE AND LACTATE DEHYDROGENASE ACTIVITIES IN HUMAN BLOOD SERUM AFTER A SINGLE, MODERATE DOSE OF VARIOUS ALCOHOLIC BEVERAGES
Roman MĄDRO1, Hanna CZEKAJSKA-ŁUCKIEWICZ1, Kazimierz PASTERNAK2
1Department of Forensic Medicine, Medical Academy, Lublin
2Department of General Chemistry, Medical Academy, Lublin
Streszczenie
The studies were performed on 12 healthy men who were given ethanol (first vodka, then wine and finally beer) in a single, oral dose of 0.84 g/kg b.w. The activities of both dehydrogenases were determined by calorimetric methods in serum collected prior to alcohol administration and when its blood level ≤ 0.1‰. During the experiment, the blood levels of ethanol and total acetaldehyde were examined. As we expected, alcohol resulted in a statistically significant increase in enzyme activities (except for isocitrate dehydrogenase after beer). Considering the remaining results, the authors conclude that an increase in serum lactate dehydrogenase may result from the impaired function of the hepatocyte membrane, while an increase in isocitrate dehydrogenase depends on the ethanol elimination rate.
Słowa kluczowe
Alcoholemia; Lactate dehydrogenase (LDH); Isocitrate dehydrogenase (ICDH); Serum levels; Alcoholic beverages.