Problems of Forensic Sciences 1998 Vol. 38 (XXXVIII) 26-35
COMPARISON OF HEAD-SPACE AND - SPME TECHNIQUES FOR DETRMINATION LOW CONCENTRATIONS ALCOHOLS IN BLOOD BY MEANS GAS CHROMATOGRAPHY METHOD
Ewa GOMÓŁKA1, Janusz PACH1, Wojciech PIEKOSZEWSKI2,3
1Clinical Toxicology, Collegium Medicum, Jagiellonian University, Cracow
2Institute of Forensic Research, Cracow
3Laboratory of Clinical Toxicology, Department of Laboratory Diagostics, L. Rydygier Regional Specialistic Hospital, Cracow
Streszczenie
A method of gas chromatography (capillary column) was worked out for determination of methyl, ethyl, isopropyl and butyl alcohols as well as acetaldehyde and acetone at low concentrations in blood. The studied compounds were separated from blood samples with headspace and head-space - SPME methods. Whilst the headspace method allowed to determine all the studied compounds at levels occuring in biological materials, the head-space - SPME method did not allow to determine acetaldehyde and small concentrations of isopropanol. In spite of its lower sensitivity the head-space - SPME method revealed a good correlation to the headspace method in the case of quantitation of methyl alcohol and a fair correlation in the case of acetone and isopropanol. The newly worked out method was succesfully applied for determination of methanol and other compounds in the case of 12 patients of Detoxication Centre, Clinical Toxicology, Collegium Medicum, the Jagiellonian University, Cracow.
Słowa kluczowe
Alcohols; Head-space; SPME; Gas chromatography.