Problems of Forensic Sciences 1997 Vol. 35 (XXXV) 129-139
RELATIONS BETWEEN WATER CONTENT IN A BLOOD SAMPLE AND ETHANOL DETERMINATION RESULTS
Roman MĄDRO1, Grzegorz BUSZEWICZ1, Hanna CZEKAJSKA-ŁUCKIEWICZ1, Andrzej KRAJKA2
1Katedra i Zakład Medycyny Sądowej Akademii Medycznej w Lublinie
2Instytut Matematyki Uniwersytetu Marii Skłodowskiej-Curie w Lublinie
Streszczenie
The paper describes an attemt to determine the correlation between the ethanol concentration (determined by two analytical methods: Widmark method, and the head-space method) and the hydration state of analysed blood samples. Water content in the blood sample (showing an initial ethanol concentration of about 1.8‰ and 3.6‰) was adjusted by changing the ratio between the erythrocytic mass and the serum. It this way, a phenomenon was simulated which occurs in corpses due to blood sedimentation. It has been determined that the radical function is the best equation to describe the correlation between the ethanol concentration and water in blood samples. A comparison of the two methods applied to determine the ethanol concentration proved that results obtained by the head-space technique depend to a less degree on the sample hydration state. It also appeared that, despite the analytical method used, the impact of the hydration state of the blood sample was stronger if higher concentrations were determined, and less strong - when lower ones were determined.
Słowa kluczowe
Ethanol determination; Water content; Blood, Head-space.