Problems of Forensic Sciences 2000 Vol. 43 (XLIII) 24-34
ANALYSIS OF VARIOUS OPIATES INCLUDING HYDROCODONE OR HYDROMORPHONE IN BIOSAMPLES BY GC/MS AFTER SILYLATION
Marie BALÍKOVÁ, Vera MARESOVÁ, Vilma HABRDOVÁ
Institute of Toxicology and Forensic Chemistry, 1st Medical Faculty and Hospital, Charles University, Prague, Czech Republic
Streszczenie
A GC/MS method suitable to conduct practical toxicological analyses of unspecified opiates including hydrocodone or hydromorphone is presented. Silylation can be a convenient reaction to derivatize unknown analytes in toxicological samples. However, silylation of drugs with tautomeric keto/enol groups in molecular structure can cause analytical problems due to poor reproducibility. After using N-methyl-N-trimethylsilyltrifluoracetamide (MSTFA) acceptable results were achieved.
Słowa kluczowe
Hydrocodone; Hydromorphone; GC/MS.