Problems of Forensic Sciences 2000 Vol. 43 (XLIII) 98-104
ELECTROPHORETIC ANALYSIS OF SERUM PROTEINS OF BIRDS AND MAMMALS
Emanuel G. E. HELAL1, Samir A. M. ZAHKOUK1, Hamdy A. MEKKAWY2
1Zoology Department, Faculty of Science, Al-Azhar University for Girls, Cairo, Egypt
2The National Center for Social and Criminological Research, Cairo, Egypt
Streszczenie
The electrophoretic patterns of the blood serum of birds such as pigeons and chickens and mammals such as Guinea pigs, cats, dogs and rabbits were checked. Various protein glycoprotein and lipoprotein bands were separated by both native and SDS methods. Chickens showed 13 bands while pigeons showed 12 bands by the native electrophoretic method for total proteins. On the other hand, both cats and dogs showed 7 bands, while Guinea pigs and rabbits showed 10 and 13 bands respectively. Total proteins by SDS methods for birds achieved 8 and 13 bands for both pigeons and chickens respectively. The SDS method revealed 13 bands for Guinea pigs and rabbits and showed 10 and 18 bands for cats and dogs, respectively. Native glycoproteins serum showed 8 bands for both pigeons and chickens. Rabbits and Guinea pigs showed 8 bands, while cats showed 10 and dogs showed 7 bands. SDS glycoproteins were significantly different between pigeons and chickens showing 4 and 10 bands respectively. The serum of Guinea pigs showed 8 bands but that of dogs and cats showed 11 and 16 bands respectively. Prestaining lipoprotein showed 2 bands for cats, dogs and rabbits. Four bands were separated for both chickens and Guinea pigs and 5 bands for pigeons. The basic data reported here represents a fingerprint peculiar specific to each animal useful in classification and evolution studies and it may be of a great importance in forensic science.
Słowa kluczowe
Electrophoresis; Glycoproteins; Lipoproteins.