Problems of Forensic Sciences 2000 Vol. 43 (XLIII) 126-130
DETERMINATION OF GAMMA-HYDROXYBUTYRATE (GHB) BY HEADSPACE-GC/MS (FID) IN FORENSIC SAMPLES
Sys Stybe JOHANSEN, Soren FELBY
Department of Forensic Chemistry, Institute of Forensic Medicine, University of Copenhagen, Copenhagen, Denmark
Streszczenie
A fast, simple, and selective method for the determination of gammahydroxybutyrate (GHB) in whole blood and urine is developed. GHB is converted to its lactonic form gamma-butyrolactone (GBL) under acidic conditions and high temperatures. The analysis is done by headspace GC with a flame ionisation detector or coupled to a mass spectrometer. The detection limit of GHB is low ppm. The method is linear using FID or MS over a range of several factors over 3 orders of magnitude in whole blood and urine. The reproducibility is below 10%.
Słowa kluczowe
GHB; GC/MS; GC.