Problems of Forensic Sciences 2000 Vol. 43 (XLIII) 250-256
SIMULTANEOUS DETERMINATION OF NALTREXONE AND 6-β-NALTREXOL IN SERUM BY HPLC
Katja SÄRKKÄ, Kari ARINIEMI, Pirjo LILLSUNDE
Laboratory of Substance Abuse, national Public Health Institute, Manerheimintie, Helsinki, Finland
Streszczenie
A reliable and simple method for the simultaneous determination of naltrexone and 6-β-naltrexol in human serum by using high-performance liquid chromatograph (HPLC) was developed. Liquid-liquid extraction with butylacetate from basic solutions (pH 9) was chosen as extraction with nalorphine as an internal standard (IS). Analytes were back-extracted from organic solvent into perchloroacid. The acid extract was chromatographed by HPLC with reverse-phase ODS-column and electrochemical detector. The mobile phase was NaH2PO4 solution with asetonitrile as an organic modifier and octanesulphonic acid and tetraethylammonium-hydrogensulphate as ion-pair reagents. The recovery of extraction method was 48% for naltrexone and 75% for 6-β-naltrexol. Limit of quantification was 5.0 ng/ml for naltrexone and 1.0 ng/ml for 6-β-naltrexol. Analysed concentrations of naltrexone differed from theoretic concentrations from 0.7 to 2.3% and those of 6-b-naltrexol 2.6%. Relative standard deviation of within-day assay was from 0.9 to 5.7% for naltrexone and from 0.8 to 4.2% for 6-b-naltrexol and between-day 5.7 and 4.2%, respectively. Our results indicate that the developed method is suitable for determination of naltrexone and 6-β-naltrexol in human serum.
Słowa kluczowe
Naltrexone; Nartrexol; HPLC.