Problems of Forensic Sciences 2000 Vol. 43 (XLIII) 263-274
DIRECT EXTRACTION OF BENZODIAZEPINE METABOLITE WITH SUPERCRITICAL FLUID FROM WHOLE BLOOD
Kenichi TAKAICHI, Shuji SAITOH, Yoshio KUMOOKA, Noriko TSUNODA
National Research Institute of Police Science, Chiba, Japan
Streszczenie
Our target is to develop a simple and a speedy extraction method of drug from biological sample. Supercritical fluid extraction (SFE) is well known as a speedy and contaminant-free extraction method. So we applied, the advantages of the SFE which has replaced a conventional extraction method, that is, liquid-liquid extraction (LLE) and solid phase extraction (SPE). In previous papers, we reported that SFE methods could be combined with freeze-drying or packing materials to reduce the problems of the LLE and the SPE. The SFE method with the packing material can directly extract drugs from biological fluid samples without the need for deproteinization. However, the recovery of the drug metabolite has not yet been considered. In this report, an evaluation has been made of whether or not the drug metabolite can be extracted from whole blood with the SFE method combined with the the packing material. The metabolite of the drug is generally a polar compound having a strong polar group such as hydroxyl (OH), desalkylamino (NH), and carboxyl (COOH) group. Therefore, the metabolite of the drug might be easily adsorbed by packing material and might not be extracted. Oxazepam was chosen as a typical metabolite of the drug (Diazepam). Quantitative analysis of the extract was carried out with gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS). The metabolite of the diazepam can be extracted as long as conditions are satisfactory in the SFE using molecular sieves 5A as a filler.
Słowa kluczowe
Supercritical fluid extraction (SFE); Benzodiazepines; Biological samples.