Problems of Forensic Sciences 2000 Vol. 43 (XLIII) 35-42

HUMAN LIVER CARBOXYLESTERASES: PURIFICATION OF THESE TWO ENZYMES, PROPERTIES IN RELATION TO COCAINE AND ALCOHOL METABOLISM, AND SPECIFICITIES

Karine BERTHOIN1,2, François WIDMER3, Christian GIROUD2, Patrice MANGIN2, Luc BARRET1
1Laboratoire de Médecine Légale et Toxicologie, UFR de Médecine, Grenoble, France
2Laboratoire de Toxicologie Analytique, Institut Universitaire de Médecine Légale, Lausanne, Switzerland
3Laboratoire de Biologie et Physiologie Végétales, Lausanne, Switzerland

Streszczenie
The purpose of this work was:
1) investigation of cocaine metabolism in association with alcohol intake thoroughly. Carboxylesterases specificities with cocaine and cocaethylene metabolites were determined by incubating one of the two purified enzymes with benzoylecgonine, ecgonine methyl ester, ecgonine ethyl ester, norcocaine and norcocaethylene;
2) determination of each enzyme affinity for various xenobiotics. The first step consisted in isolating carboxylesterases 1 and 2 from human liver obtained by autopsy.

Słowa kluczowe
Cocaine; Ethanol; Cocaethylene; Carboxylesterases.

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