Problems of Forensic Sciences 2000 Vol. 42 (XLII) 143-151
THE DEVELOPMENT OF A LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY METHOD FOR THE DETECTION OF ALLOPURINOL AND ITS METABOLITE IN HUMAN HAIR
Tamsin KELLY1, Harry ROSE1, Jim KEEGAN1, Ken WILLIAMS2, Costa CONN1
1Department of Chemistry, Materials and Forensic Sciences, University of Technology, Sydney, Australia
2Clinical Pharmacology, St. Vincent's Hospital, Darlinghurst, Australia
Streszczenie
The preliminary development of a liquid chromatography-mass spectrometry (LC/MS) method for the detection of allopurinol, and its metabolite, oxypurinol in human hair is reported. The experimental results indicate that allopurinol is not present in incorporated hair, while oxypurinol is. Furthermore, the results indicate that the developed LC/MS method may provide a means by which clinical monitoring of oxypurinol can be achieved; an appplication of potential importance due to the adverse effects associated with the occurmulation of oxypurinol in the body. The LC/MS method described is the first to detect these analytes in hair, and among the first methods to utilise LC/MS for the analysis of drugs in hair.
Słowa kluczowe
Allopurinol; Oxypurinol; Hair analysis; LC/MS.