Problems of Forensic Sciences 2001 Vol. 47 (XLVII) 314-321
ADDITIONAL VARIABILITY AT THE D21S11 LOCUS: SEQUENCING EVIDENCE OF A NEW ALLELE D21S11*33.1
Anita DETTLAFF-KĄKOL1, Ryszard PAWŁOWSKI1,2
1Department of Forensic Medicine, Medical University of Gdańsk, Gdańsk, Poland
2Institute of Forensic Research, Cracow, Poland
Streszczenie
Analysis of short tandem repeats (STR) is rapidly growing method of individual identification in forensic testing. The highly polymorphic STR locus D21S11 was investigated in a Polish population sample by PCR amplification using commercially available AmpFISTR Profiler Plus Kit (Perkin Elmer) and capillary electrophoresis on ABI Prism 310 Genetic Analyzer. For fragment sizing, PCR amplification product and allelic ladders were run with an internal size standard ILS400 labelled with CXR dye. During routine testing of reference samples a new variant of allele 33 was found. GeneScanTM analysis showed the presence of off-ladder allele (218.53 bp) exactly one nucleotide shorter than allele 33.2 (219.60 bp). The variant allele was sequenced in both directions and the following repetitive sequence was found: (TCTA)5 (TCTG)6-CR-(TCTA)13-A-TCTA. According to the ISFG recommendations the allele was designated 33.1. Frequencies of D21S11 alleles in a population sample of over 900 unrelated individuals originating from Polish population are presented.
Słowa kluczowe
D21S11; Short tandem repeats; DNA polymorphism; Sequence structure.