Problems of Forensic Sciences 2001 Vol. 45 (XLV) 16-35
STABILITY OF COCAINE IN BLOOD AND IN OTHER TISSUES*
Marianna KISZKA, Grzegorz BUSZEWICZ, Roman MĄDRO
Chair and Department of Forensic Medicine, Medical Academy, Lublin
Streszczenie
The stability of cocaine (C) in blood samples and in homogenised samples of liver, kidney and brain was tested. The influence of time (up to 90 days) and temperature (+25°C, +4°C, –20°C) were evaluated, and, in the case of blood, the influence of acetic acid and sodium fluoride as well. For isolation of C and benzoylecgonine the solid phase extraction (SPE) was performed. Quantitative analysis was carried out with the use of the HPLC method. It was shown that the stability of C increased with lowering the temperature of storage of the biological material. Freezing blood and other tissues down to –20°C made C stable up to 90 days. In blood samples stored at +4°C a similar stability of C was reached by addition of acetic acid (to pH = 5) and NaF.
Słowa kluczowe
Cocaine; Benzoylecgonine; Stability; Autopsy material; Blood; Liver; Kidney; Brain.