Problems of Forensic Sciences 2001 Vol. 47 (XLVII) 399-404
HLA-DR TYPING OF GENOMIC DNA
Zofia SZCZERKOWSKA, Joanna WYSOCKA
Institute of Forensic Medicine, Medical University, Gdańsk, Poland
Streszczenie
Advances in molecular biology techniques allowed for introduction of PCR- based methods for HLA typing. Due to PCR – based DNA amplification genetic identification has become available. The aim of this paper was to present the results of investigation high polymorphic HLA-DR locus by PCR-SSP method. Blood and tissues samples (muscles and brains) were obtained from 50 corpse adult and bimonthly fetus from North part of Poland. The post-mortem periods ranged from 2 to 7 days. DNA was extracted also from older – two years tissues. Genomic DNA was isolated by two methods: using phenol/chloroform extraction and “Easy Tissue DNA Prep.” kit (A&A Biotechnology). HLA-DR alleles were typed using DYNAL DR “ low resolution” SSP kit. PCR products were separated on agarose gel with ethidium bromide and estimated in ultraviolet light. Phenotypic and genotypic rates of blood and tissues has been analysed. In our materials 6 alleles were detected. The high frequency of HLA-DR 6 and HLA-DR 5 alleles have been found. Rare HLA-DR 1 allele was observed. We found PCR-SSP HLA typing method very useful in routine HLA-DR identification.
Słowa kluczowe
HLA-DR; PCR-SSP; HLA class II.