Problems of Forensic Sciences 2002 Vol. 49 (XLIX) 14-37
EVALUATION OF THE METHOD OF COCAINE AND BENZOYLECGONINE ISOLATION FROM POST-MORTEM MATERIAL. PART II: SOLID PHASE EXTRACTION (SPE)
Marianna KISZKA, Roman MĄDRO
Chair and Department of Forensic Medicine, Medical Academy, Lublin
Streszczenie
Cocaine (C) and benzoylecgonine (BE) were isolated from water solutions, blood, urine and homogenates of the liver, kidney and brain. The columns used were: C-18 (Baker), Cyano (CN) (Baker) and Bond Elut Certify (Varian). The eluent was a mixture of dichloromethane – isopropanol – ammonium hydroxide 80:20:2. The highest recovery of xenobiotic extraction from urine (BE about 80% and C over 90%) and from blood (about 67% of BE and 83% of C), and good recovery from tissues (44–58% of BE and 66–72% of C) was achieved using Bond Elut Certify columns. Such extracts from “fresh” autopsy material showed low “background” levels, whichenabled their further analysis using the HPLC method. Additionally, the effects of various factors on the extraction yield using B. E. Certify columns were studied. No significant effects of pH and the amount of the solvent on the recovery of C and BE were observed. It was found that the recovery of both xenobiotics from the brain homogenates increased after diluting them with a higher amount of phosphate buffer and decreased when the samples were prepared using acetonitrile.
Słowa kluczowe
Cocaine; Benzoylecgonine; Solid phase extraction; Autopsy material.