Problems of Forensic Sciences 2002 Vol. 49 (XLIX) 74-83
EVALUATION OF (CA)n DINUCLEOTIDE MARKERS IN PATERNITY ANALYSIS
Anitha A., Moinak BANERJEE
Rajiv Gandhi Centre for Biotechnology, Jagathy, Kerala, India
Streszczenie
Developing robust molecular markers for forensicDNAfingerprinting is an important issue, as stringent criteria for their selection and use have to be met. In our study, the effectiveness of several (CA)n dinucleotide molecular markers was evaluated in several random individuals from various ethnic backgrounds of Kerala, for paternity analysis. We used the most advanced technique of PCR genotyping, using microsatellite markers. Allele patterns were detected by electrophoresis of the PCR products on a 6% denaturing polyacrylamide gel, followed by autoradiography. The heterozygosity values of D9S1847, D9S1813, D10S558 and D22S4461 microsatellite markers in the representative populations were in the range of 0.84 to 0.89. These loci were found to be highly effective for paternity analysis and have great potential to be developed into markers for probability searches of forensic samples.
Słowa kluczowe
Paternity; Microsatellites; Alleles; Heterozygosity.