Problems of Forensic Sciences 2004 Vol. 60 (LX) 104-116
PERSONAL IDENTIFICATION BASED ON NUCLEAR DNA EXTRACTED FROM BONES OF DECEASED INDIVIDUALS
Ewa KAPIŃSKA, Zofia SZCZERKOWSKA
Chair and Department of Forensic Medicine, Medical University of Gdańsk, Gdańsk
Streszczenie
Personal identification with the use of skeletal remains (bones) is one of the most important analyses in forensic medicine. The environment to which the material has been exposed (high temperature, sea and river water or dry air) has a crucial influence on the success of the investigation. We examined bones obtained from bodies that had been partially or completely decomposed and partially burned. In this paper we describe an evaluation of four different DNA extraction methods: phenol-chloroform, silica, sodium acetate and the DNA IQTM System (Promega). The extracts were purified and concentrated in Microcon 30/100. DNA was quantified and profiled by AmpFlSTR®IdentifilerTM or AmpFlSTR®SEfilerTM PCR Amplification Kit (Applied Biosystems). In all cases, full profiles were obtained after phenol-chloroform extraction. DNA isolation using silica and a commercial kit (Promega) gave a full STR genotype only in the case of the burned body and partially after amplification of DNA from the unburied corpse (exposed to dry air). However, no positive signals of PCR products were observed from bodies submerged in water. No good results of amplification were obtained after sodium acetate extraction of DNA from bones.
Słowa kluczowe
Bones; DNA extraction; Personal identification; AmpFlSTR®IdentifilerTM; AmpFlSTR®SEfilerTM.