Problems of Forensic Sciences 2005 Vol. 63 (LXIII) 306-315
ANALYSIS OF SELECTED PARAMETERS OF OXIDATIVE STRESS AND THE ANTIOXIDATION SYSTEM IN HEART HOMOGENATES AND BLOOD IN EXPERIMENTAL CHRONIC INTOXICATION WITH ETHANOL
Ewa MEISSNER1, Maciej BARZDO1, Agnieszka P. JURCZYK1, Beata JANKOWSKA1, Dariusz NOWAK2, Jarosław BERENT1, Leszek MARKUSZEWSKI1, Stefan SZRAM1
1Chair and Departament of Forensic Medicine, Medical University, Łódź
2Chair and Departament Experimental and Clinical Physiology, Medical University, Łódź
Streszczenie
Metabolism of alcohols, including ethanol, generates oxidation stress. The undertaken investigations aimed at following changes in the oxidation-antioxidation balance in the heart and blood of rats chronically intoxicated with 1 M ethanol solution. The study was performed on 30 male Lewis rats, aged around 6 months (240–290 g) receiving ethanol for 4, 8 and 12 weeks. Ten rats that were given tap water constituted a control group. At the 8th week of intoxication with ethanol, a nearly three-fold increase in thiobarbituric acid reactive substances (TBARs) concentration in heart homogenates was observed compared to controls and the 4-week group. At the 12th week of the experiment there was a decrease in TBARs concentration in comparison with the concentration in the 8th week. Biochemical investigations revealed an elevation of TBARs concentration in erythrocytes of rats chronically intoxicated with ethanol at each time interval (4, 8, 12 weeks) in relation to the control group. At the 4th week of intoxication, a rise in hydrogen peroxide in myocardium homogenate was found along with a declined concentration of free thiol group as compared to the control group. During rat intoxication with 1 M ethanol there was an observed increase in catalase activity at the 4th week of the experiment. In the 8th week, this activity dropped to values lower than in the controls. At the 12th week, the catalase activity was elevated as compared to the 8th week, but was still lower than the control value. The peroxide dismutase activity was decreased in comparison with the control group.
Słowa kluczowe
Lipid peroxidation; Ethanol, Dismutase; Catalase; Hydrogen peroxide; Free SH groups.