Problems of Forensic Sciences 2005 Vol. 62 (LXII) 188-203
DETERMINATION OF THC, THC-COOH, CBN AND CBD IN HAIR WITH APPLICATION OF DERIVATISATION AND CHROMATOGRAPHIC TECHNIQUES
Marek WIERGOWSKI1, Marzena PATELCZYK2, Katarzyna GALER1, Beata SZPIECH1
1Chair and Department of Forensic Medicine, Medical University, Gdańsk
2Chemical Faculty, University of Technology, Gdańsk
Streszczenie
In the study, analytical conditions for the determination of selected cannabinols by GC-FID and HPLC-UV methods were optimised and an attempt was made to develop a determination procedure for these compounds in hair. Application of MTBSTFA derivatisation of cannabinols in the GC-FID procedure resulted in much lower limits of detection. For hair sample preparation in the HPLC-UV procedure, ultrasonic-assisted extraction (UAE) and dichloromethane as an extrahent were chosen. The best chromatographic separation of cannabinols was achieved by HPLC-UV in isocratic elution with acetonitrile and 0.05 mmol/dm3 formic acid (75:25, v/v). For the developed procedure which utilised UAE-HPLC/UV, the evaluated limits of detection in hair were: 3.4 µg/g for THC, 0.2 µg/g CBN, 0.5 µg/g CBD and 0.4 µg/g for THC-COOH. The procedure was applied to the determination of cannabinols in authentic hair samples (n = 12), and the average concentrations in 1 g of hair were: 3.4 µg THC (n = 1); 1.4 µg CBD (n = 7); 0.5 µg CBN (n = 11) and 2.1 µg THC-COOH (n = 12).
Słowa kluczowe
Cannabinols; Hair analysis; Derivatisation; Liquid chromatography; Gas chromatography.