Problems of Forensic Sciences 2010 Vol. 81 (LXXXI) 91-100
EVALUATION OF THE AGILENT 2100 BIOANALYZER AS A TOOL FOR DNA ANALYSIS IN FORENSIC GENETICS
Marta GORZKIEWICZ, Anna DULEBA, Edyta RYCHLICKA, Marcin WOZNIAK, Tomasz GRZYBOWSKI, Karol OELIWKA
Chair of Legal Medicine, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, Poland
Streszczenie
Quantitation and qualification of DNA is crucial for DNA profiling in forensic cases. In this report, we have evaluated the application of the 2100 Bioanalyzer to these initial steps of DNA analysis. On the basis of electropherograms, sizing and quantitation
of DNA isolated from refer ence material and real biological traces was performed. The concentration values obtained using the 2100 Bioanalyzer were compared to Quantifiler results. We concluded that the 2100 Bioanalyzer can be used to analyse a large
amount of nondegraded genomic DNA. However, this system is not suitable for analysis of forensic samples.
Agilent 2100 Bioanalyzer; Quantitative and qualitative analysis; Genomic DNA